ISSN: 0034-8376
eISSN: 2564-8896
eISSN: 2564-8896
VOLUME 67 - NUMBER 4 / July - August (Original articles)
Ãngel Antonio Vértiz-Hernández, Coordinación Académica Región Altiplano, Universidad Autónoma de San Luis PotosÃ, Matehuala, S.L.P., México
Flavio MartÃnez-Morales, Faculty of Medicine, Universidad Autónoma de San Luis PotosÃ, S.L.P., México
Roberto Valle-Aguilera, Faculty of Medicine, Universidad Autónoma de San Luis PotosÃ, S.L.P., México
Pedro López-Sánchez, School of Medicine, Instituto Politécnico Nacional, México, D.F., México
Rafael Villalobos-Molina, Iztacala School of Graduate Studies, National Autonomous University of Mexico (FES-I.-UNAM), Tlalnepantla, Edo. Mexico; National Laboratory in Health, Molecular Diagnosis and Environmental Effect in Chronic-Degenerative Diseases, UNAM, Tlalnepantla, Edo. Mex., Mexico;
José Pérez-Urizar, Faculty of Advanced Studies-Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Edo. de México, México
Background: Cyclooxygenase-2 selective inhibitors have been developed to alleviate pain and inflammation; however, the use of a selective cyclooxygenase-2 inhibitor is associated with mild edema, hypertension, and cardiovascular risk. Aim: To evaluate, in an experimental model in normotensive rats, the effect of treatment with parecoxib in comparison with diclofenac and aspirin and L-NAME, a non-selective nitric oxide synthetase, on mean arterial blood pressure, and cyclooxygenase-1 and -2 messenger RNA and protein expression in aortic tissue. Methods: Rats were treated for seven days with parecoxib (10 mg/kg/day), diclofenac (3.2 mg/kg/day), aspirin (10 mg/kg/day), or L-NAME (10 mg/kg/day). Mean arterial blood pressure was evaluated in rat tail; cyclooxygenase-1 and -2 were evaluated by reverse transcription-polymerase chain reaction and Western blot analysis in aortic tissue. Results: Parecoxib and L-NAME, but not aspirin and diclofenac, increased mean arterial blood pressure by about 50% (p < 0.05) without changes in cardiac frequency. Messenger RNA cyclooxygenase-1 expression in aortic tissue was not modified with any drug (p < 0.05). L-NAME and parecoxib treatment decreased messenger RNA cyclooxygenase-2 and cyclooxygenase-2 (p < 0.05). While cyclooxygenase-1 protein decreased with the three drugs tested but not with L-NAME (p < 0.05), the cyclooxygenase-2 protein decreased only with aspirin and parecoxib (p < 0.05). Conclusion: Parecoxib increases the blood pressure of normotensive rats by the suppression of COX-2 gene expression, which apparently induced cardiovascular control.
Keywords: Parecoxib. Hypertension. Cyclooxygenase-2. Prostaglandin I2. Thromboxane A2. mRNA inhibition.
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